Partial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
author
Abstract:
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the substrates tested, pyrogallol was the best substrate for PPO with a Km value of 1.5 mM. The optimum pH for PPO activity was found to be 6.8. The enzyme showed high activity over a broad pH range of 4 - 8. The optimal pH and temperature for enzyme activity were found to be 6.8 and 50-60 °C, respectively. km value for tomatoe PPO is calculated 25 mM for catechol and 1.5 mM for pyrogallol and 8.5 mM for L-dopa. As can be seen, affinity of PPOs for various substrates varies widely. The enzyme showed a broad activity over a broad pH and temperature range. The thermal inactivation studies showed that the enzyme is heat resistant. The enzyme showed the highest activity toward pyrogallol and no activity toward tyrosine. Of the inhibitors tested, the most potent inhibitors was sodium kojic acid
similar resources
partial purification and characterisation of polyphenol oxidase from tomatoes (solanum lycopersicum)
polyphenol oxidase (ppo) from tomatoes was extracted and partially purified through (nh4)2so4 precipitation, dialysis and ion exchange chromatography. the activity of polyphenol oxidase was investigated in solanum lycopersicum. spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (vmax) and michealis - menten constant (km). of the ...
full textEffect of Sodium Dodecyl Sulphate on Partial Purified Polyphenol Oxidase Activity in Red and Green Tomatoes (Solanum Lycopersicum)
In order to better understand how to prevent enzymatic greenning, it is important to understand kinetic properties of polyphenol oxidase. we investigated the effect of SDS on the rate of catechol oxidation by small cherry tomato partial purified PPO. PPO activity increased with increasing SDS concentration. The most effective concentration of SDS was 0.8, 1 and 1.25 mM in according to pH and ...
full textEffect of Sodium Dodecyl Sulphate on Partial Purified Polyphenol Oxidase Activity in Red and Green Tomatoes (Solanum Lycopersicum)
In order to better understand how to prevent enzymatic greenning, it is important to understand kinetic properties of polyphenol oxidase. we investigated the effect of SDS on the rate of catechol oxidation by small cherry tomato partial purified PPO. PPO activity increased with increasing SDS concentration. The most effective concentration of SDS was 0.8, 1 and 1.25 mM in according to pH and ...
full texteffect of sodium dodecyl sulphate on partial purified polyphenol oxidase activity in red and green tomatoes (solanum lycopersicum)
in order to better understand how to prevent enzymatic greenning, it is important to understand kinetic properties of polyphenol oxidase. we investigated the effect of sds on the rate of catechol oxidation by small cherry tomato partial purified ppo. ppo activity increased with increasing sds concentration. the most effective concentration of sds was 0.8, 1 and 1.25 mm in according to ph and ...
full textPurification and Characterization of Polyphenol Oxidase from Glandular Trichomes of Solanum berthaultii.
Type A glandular trichomes of the wild potato (Solanum berthaultii Hawkes) entrap insects by rapidly polymerizing the trichome contents after breakage by insect contact. Polymerization of trichome exudate appears to be driven by a soluble polyphenol oxidase (PPO). PPO constitutes up to 70% of the protein in individually collected trichomes and reaches a concentration approaching 200 mum in thes...
full textMy Resources
Journal title
volume 1 issue 6
pages 637- 648
publication date 2013-06-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023